9/25/2023 0 Comments Western blot hiv testHowever, there have been no large-scale studies that can provide any significant findings from the band patterns of WB tests. ,, Īlthough the overall sensitivity and specificity of the WB for detection of antibodies to the various viral proteins is high, there have been substantial differences in the timing of the appearance of antibody bands and their intensities during different stages of HIV infection. Western blotting is one of the reference confirmatory tests for the diagnosis of HIV infection or after inconclusive EIA results. HIV tests have become progressively more sensitive and specific with the use of recombinant and/or synthetic peptide antigens as the basis of the tests. Conclusion: Interpretation of the WB band pattern in combination with clinical features may be occasionally useful in predicting the stage of HIV infection.Ĭorrelation of various western blot band patterns with the WHO stages categorized into three HIV infection stages (N=1,467)ĬD4 cell counts as observed in the uncommon western blot band patternsĬases with the absence of reactivity towards various proteins on western blot testĬurrent laboratory diagnosis of human immunodeficiency virus (HIV) infection is mainly based on the detection of anti-HIV antibodies. Correlation of CD4 cell counts with the various uncommon band patterns showed that only 5.56% (4/72) had counts in the 200-500 cells/�l range, whereas 45.83% and 48.61% had counts of 500 cells/�l respectively. On classifying the WB reactive cases by the WHO clinical staging system, 38.45% (564/1467) were in Stage 1, 47.99% (704/1467) in stages 2 and 3 and 13.56% in stage 4. Of all individual bands, p31 band was the most frequently missing one, absent in 7.09% cases. Results: Reactivity with all the bands was the most commonly observed WB pattern, occurring in 92.91% (1363/1467) of cases, whereas the other 7.09% showed uncommon band patterns. For the interpretation of the WB results in this study, the guidelines recommended by NACO, India were followed. Methods: We performed a retrospective study with 1,467 HIV-1 infected cases confirmed by WB test between January 2002 to July 2005, with the objective of mapping different band patterns of western blot results and determining whether the presence or absence of certain bands was associated with any specific stage of HIV infection. Aims: Mapping different band patterns of Western blot results and correlating them with stages of HIV infection. Abstract Background: Although the overall sensitivity and specificity of the western blot (WB) test for detection of antibodies to various viral proteins is high, there has been a substantial difference in the timing of the appearance of antibody bands and their intensities during different stages of HIV infection.
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